Molecular “eyewitness”: Forensic prediction of phenotype and ancestry. The Use of Forensic DNA Phenotyping in Predicting Appearance and Biogeographic Ancestry. Forensic genetic analysis of bio-geographical ancestry. Forensic DNA Phenotyping: Predicting human appearance from crime scene material for investigative purposes. The results suggest that this MPS assay is a useful tool for basic appearance and ancestry prediction in forensic genetics for users interested in applying PowerSeq chemistry and MiSeq for this purpose.īGA DNA phenotyping EVC prediction HIrisPlex-S PowerSeq VISAGE ancestry appearance forensic DNA phenotyping phenotype prediction. It was robust in terms of repeatability and concordance and provided useful results with casework-type samples. This targeted MPS assay provided complete genotypes at all 153 SNPs down to 125 pg of input DNA and 99.67% correct genotypes at 50 pg. The assay was evaluated for sensitivity, repeatability and genotyping concordance, as well as its performance with casework-type samples. The panel consists of 153 single nucleotide polymorphisms (SNPs) that provide information about EVCs (41 SNPs for eye, hair and skin color from HIrisPlex-S) and continental BGA (115 SNPs three overlap with the EVCs SNP set). Here, we present an evaluation of the VISAGE Basic tool for appearance and ancestry prediction based on PowerSeq chemistry (Promega) on a MiSeq FGx System (Illumina). The EU-funded VISAGE (VISible Attributes through GEnomics) Consortium has developed various targeted MPS-based lab tools to apply FDP in routine forensic analyses. Technical developments in Massively Parallel Sequencing (MPS) enable the simultaneous analysis of hundreds of DNA markers, which improves successful Forensic DNA Phenotyping (FDP). The study of DNA to predict externally visible characteristics (EVCs) and the biogeographical ancestry (BGA) from unknown samples is gaining relevance in forensic genetics.
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